#6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. their consistent performance, reliability and durability. . SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. . Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Microplate Readers BioTek Epoch 2 Microplate Reader. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Highly customizable low volume. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Compact designs. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Table of Contents. . Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Contents of wells were pipetted up and down several times to mix. Access this by going to Help >. . The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The SpectraMax 190 reader can be integrated with Molecular . limit of detection (LLD) for ATP was estimated for each microplate reader using the . 2 software: see SoftMax® Pro 7. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. 1 software Download page. No accessories are needed and SoftMax® Pro Software. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Also see:. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Cell Cytotoxicity assay procedure. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Next generation SpectraMax® absorbance microplate readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. An assay plate containing a 15-point standard curve was read on the SpectraMax. Figure 2-23: Bottle holder To install the bottle holder: 1. Molecular Devices SpectraMax M2 Multimode Plate Reader. To resume collecting kinetic measurements within the same file, press the Append button. 0200-6263. Figure 1. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Maximum signal with lowest background. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. With optimized reagents, option to operate the. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Use the provided 3. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. With optimized reagents, option to operate the. Detection modalities include absorbance (UV-Vis Abs) and. limit of detection (LLD) for ATP was estimated for each microplate reader using the . 315 can't find zero order. 0 out of 5 $ 11,999. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 1. Additionally, user-installable options allow the SpectraMax i3 System to. 7°C (gradient heating design). no. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The temperature in the plate chamber is ambient and isothermal. Prerequisites: SoftMax Pro 7. Self Service Available. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The plate reader can measure multiple readouts, including UV. Molecular Devices Spectramax iD3 Microplate Reader. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. If it looks fine, then proceed. Blank subtraction and standard curve fitting were done automatically by the software. Other technologies such as imaging. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax Glo Steady-Luc Reporter Assay Kit. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Use similar settings for other Molecular Devices readers with fluorescence detection mode. Thank you!. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Lysozyme contains two tryptophan residues that. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. SpectraMax Microplate Readers. In . Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. microplate reader. Or, with the reader specifications noted from the Instrument Description section of your reader's. . Create custom fluorescence assays without having to purchase expensive filter sets. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. This unlocks the transport slide. 316 grating motor driver faulty. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Molecular Devices: Molecular Devices. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The Gemini EM can sample a variety of formats, from 6- to 384-well. Data exemplifying linear ranges from the three different kits are presented in Figure 2. Cell Cytotoxicity assay procedure. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. The drawing shows a standard microplate of 15 mm height covered with a lid. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. For a higher . A. Guides: SpectraMax Mini Installation Guide. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. Omnia® Compatible Microplate Reader Documentation Version No. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. SpectraMax M5 reader settings are shown. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. SpectraMax iD5 Multi-Mode Microplate Reader. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Positions H21-K22 contained plate blanks of PBS only. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The guide also covers the software features, the specifications, and the accessories of the systems. 1. Add to Cart. Summary of Contents for Molecular Devices SpectraMax M2. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Materials. Wide range of applications The VersaMax reader covers. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. 003 fmol 0. Click SoftMax Pro Software User Guide. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. The plate. It also integrates with the. Instrument settings used with the SpectraMax M5 Microplate Reader. SpectraMax 250 User. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. flexibility of monochromator-based systems. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Find regional support. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Products : General, Microplate Readers. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Detailed specifications of each model reader are reported in Section. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The price range of a reader starts at 2. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The plate reader can measure multiple readouts, including UV and visible absorbance. Add to Cart. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . , a. both cuvette-based and microplate reader applications on the same instrument. Reporter Assay. Microplate Readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 1. . The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. zip. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 303 unable to cal dark current. 2 only: SpectraMax Mini Update. Products. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. The plate reader optics are tunable for. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Temperature control. Wide range of applications. Other technologies such as imaging. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. In . User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® ABS Plus Microplate Reader can run . Type: Clear: Add to Cart. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. It was also read on the monochromator-based. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. In this product video, see how the SpectraMax M5 quickly reads samples. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. begin reading microplates with seven mouse clicks. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. Figure 1. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The chamber contains springs that position the cuvette in the proper alignment for a read. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. Category 2 and 3 microplate readers use gradient heating as well. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). See SpectraMax® Microplate Reader User Manual Download Page. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Examples: “Press carefully to engage,” “ Do not. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Using the. e. We would like to show you a description here but the site won’t allow us. Example: [Drawer]. The instrument uses a paired monochromator and xenon flash. \ Figure 3. Figure 1. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. 2. SpectraMax Microplate Readers. ELISA Plate Readers / Microplate Readers. The dual monochromator optics allow the widest range of. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. HTRF-compatible SpectraMax Multi-Mode Readers. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 0 out of 5 $ 23,995 $ 22,495. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Download PDF. Figure 6: Transcreener ADP2 FI assay principle. Please refer to the Obsolete Microplate Reader Models for more information. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. With optimized reagents, option to operate the. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. 0 out of 5 $ 11,999. San Jose, Calif. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Turn the reader off by pulling the power cord. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Ease of use. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Or scan up to 384 wells at any time to determine the best assay conditions. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. 58% for the detection module and 2. Align the two slots on the bottle holder with the two pegs on the rail. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. 20 μL of Component A was added to each well of the microplate. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Temperature control and shaking come as standard. Italic and boldface type are used for emphasis. 76358-624. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 470 nm). SAN JOSE, CA — Molecular Devices, LLC. Five preferred detection modes. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Figure 3. . Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. For a higher . Cuvette Microplate wells. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. SpectraMax ® M2 Multi-Mode Microplate Reader. It measures the optical density (OD) of samples in both 96- and 384-well. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Lift up the lid over the chamber to insert or remove a cuvette. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. 030 fmol Fluorescence Polarization (FP). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. For more sample throughput, both readersSupplier: Molecular Devices. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Fully tested with validations, warranty. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. 2. For readers without a cuvette port, these options are not shown in the software. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Plate chamber design of the SpectraMax category 1 microplate readers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The SpectraMax® iD3 Multi Mode Microplate . These lab workhorses are used in drug discovery. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The cuvette must be in place when you read the plate. 1) Select the correct plate and the wells that you want to measure. Fluorescence Microplate Reader Comparison Chart. 3) At the bottom of the strip, click on "Show. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. 0 out of 5 $ 2,999-20%. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. Find regional support. We would like to show you a description here but the site won’t allow us. Contact our support team with your questions regarding Molecular Devices products and applications. Multimode Microplate Reader.